ࡱ> a  bjbjڥڥ 8R\R\D $$$8\$_)\^Z"|||((((((($*q-(9(||4)> > > ^||J'p> (> > ::%,%|^f% 6'/)0_)p%.5.l5.%5.%> (( _)5. > : USF Research Integrity & Compliance Biosafety Level 2 Inspection Form PRINCIPAL INVESTIGATOR:  FORMTEXT       INSPECTION DATE:  FORMTEXT      BUILDING/ROOM:  FORMTEXT      INSPECTOR(S):  FORMTEXT       LAB GUIDE(S):  FORMTEXT        Biosafety Level 2YesNoN/AComments A. Standard Microbiological Practices 1. Access limited when experiments in progress.  2. Persons wash hands after work w/cultures & removing gloves, before leaving lab.  3. Eating, drinking, storing food, etc. prohibited.  4. Mouth pipetting prohibited; pipettors used.  5a. Sharps handling policies in place. 5b. Sharps disposed in biohazardous Sharps containers. 5c. Broken glassware is only handled by mechanical means. 5d. Plastic ware is substituted for glassware whenever possible. 5e. Disposable needles are not bent, sheared, broken, recapped, removed from disposable syringes, or otherwise manipulated prior to disposal. 5f. Syringes that re-sheath the needle or needleless systems are used when appropriate.  6. Splashes & aerosols are minimized.  7a. Work surfaces disinfected after completion of work and after any spill. 7b. Biohazard spill cleanup kit available. 8. Regulated waste disposed properly.  9. A biohazard sign, PI/Emergency contact information, and biosafety level are posted on entry doors to lab. 10. Insect & rodent control program in place. 11. PI ensures personnel receive appropriate training. B. Special Practices: Yes No N/A Comments 1. Laboratory staff is advised of hazards.  2. Laboratory staff is provided medical surveillance and appropriate immunizations if applicable.  3. Policy in place regarding baseline serum for at risk personnel, as appropriate.  4. A laboratory-specific biosafety manual must be prepared and adopted as policy.   5. The PI must ensure that laboratory staff demonstrates proficiency in standard and special microbiological practices.  6. Infectious agents must be placed in a durable, leak proof container during collection, handling, storage and transport.  7. Policies for containing and decontaminating spills are in place. Laboratory equipment decontaminated after spills, before repair, maintenance, or removal from lab.  8. Policies for accidental exposures are in place.   9. Animals & plants not involved in work not permitted in lab. 10. Experimental procedures that generate aerosols should be conducted within the biosafety cabinet.  11. Equipment and storage areas for use with biohazard are properly labeled. Agents are properly labeled. C. Safety Equipment (Primary Barriers) Yes No N/A Comments 1. Biosafety cabinet is certified annually.  2a. Lab coats/gowns designated for lab use is worn by all personnel for work with biohazardous agents. 2b. Remove lab coats/gowns before leaving for non-lab areas. 2c. Dispose Lab clothing appropriately or deposit for launder on-site. Lab coats/gowns are not taken home for laundering.  3. Eye and Face protection is available for procedures with potential of aerosols/splashes generation with biohazardous agents that is not conducted in the BSC cabinet or other containment device.  4a. Gloves must be worn when working with Biohazardous agents. 4b. Gloves must not be worn outside the laboratory in non-lab areas. 4c. Change gloves when contaminated or integrity compromised. 4d. Do not wash or reuse disposable gloves. 5. Eye, face and respiratory protection should be used in rooms containing infected animals, as determined by risk assessment. D. Laboratory Facilities (Secondary Barriers) Yes No N/A Comments 1. Laboratory doors should be self-closing and have locks.  2. Labs have hand wash sink. 3. Lab is easily cleaned. No carpets or rugs. 4. Lab furniture is sturdy. Spaces accessible for cleaning. Bench top impervious to water and resistant to chemicals. Chairs used in laboratory covered with a non-porous material. 5. Windows that open to exterior not recommended. If they do open to the exterior, must be fitted with fly screens. 6. BSCs located away from doors, heavily traveled areas, etc, to maintain airflow. 7. Vacuum lines protected by disinfectant traps or HEPA filters or equivalent. 8. Eyewash readily available. 9. Recommended inward flow of air without recirculation to spaces outside of the lab. 10. Air from class II BSC can be re-circulated into the lab if cabinet tested and recertified annually or can also be connected to lab exhaust system by either thimble (canopy) or direct (hard) connection. 11. Illumination is adequate, avoiding glares and reflections that could impede vision. E. Toxin Use Items Yes No N/A Comments 1. Laboratory personnel are trained use of the specific toxin.  2. Toxin specific Chemical Hygiene Plan must be available in the lab. 3. Inventory System in place to account for toxin use and disposition. 4. Toxins are stored in locked access box. 5. When toxins are in use, the room must be clearly posted Toxins in UseAuthorized Personnel Only. Unrelated and nonessential work should be restricted from areas where stock solutions of toxin or organisms producing toxin are used. 6. A certified BSC, chemical fume hood or glove box is available for manipulation of the dry toxin. F. Institutional Biosafety Committee Yes No N/A Comments 1. IBC review and approval of agent(s).  2. Changes/modifications reported to IBC.  3. USF Biosafety training for all staff in date. 4. If Shipping biohazardous agents, appropriate DOT/IATA training completed and applicable permits in place. 5. Reviewed lab spaces to determine that they are complete and accurate. 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A2 C$%,vBroken glassware is only handled by    #2 +%,vmechanical means    2 %,v.  2 %,v    2 +%,v   2 +%,v5d.  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